How to Calculate Rf Values
To do this running your sample on the same gel with molecular weight standards is necessary. How to calculate the Rf of a spot based on the distance it traveledExplore TLC with an interactive simulator.
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The word comes from chromatography when it was discovered that a given.
. The R f retardation factor value is the ratio of the solutes distance travelled to the solvents distance travelled. The following Rf values were determined. The formula for calculating the Rf values is R f Distance moved by the molecule location of the spot Distance moved by the mobile phase solvent.
R f Migration distance of the protein. Two pigments with the same Rf value are likely to be. A TLC plate showed two spots A and B with Rf 020 and 030 respectively.
The Rf values indicate how soluble the particular pigment is in the solvent by how high the pigment moves on the paper. Sketch a TLC chromatogram with the above Rf values. In paper chromatography the use of Rf values and locating agents should be covered.
This was an example that we used in class after using a ransom note created by me to figure out w. To calculate an Rf value divide the distance travelled by the component in other words the distance from the starting pencil line to the coloured spot -by the distance travelled. In western blot calculating the R f value is useful because it allows the molecular weight of a protein to be determined.
Thanks for watching this videoFor more videos httpsyoutubec_5g2xA94Hw - vb theory httpsyoutubeAbidjiAzzl8 - uv visible spectroscopy lamda max cal. How to calculate Rf values for an ink pen chromatography. The Rf value can be used to calculate the molecular weight of the protein of interest.
The solute migration value is compared by the solvent front migration value to calculate the Rf value which gives just a ratio number. How do you calculate the Rf values. What are the advantages of calculating.
To calculate an Rf value divide the distance travelled by the component in other words the distance from the starting pencil line to the coloured spot -by the distance travelled.
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